Analysis of CDS-located miRNA target sites suggests that they can effectively inhibit translation

CDS-located binding sites transiently inhibit translation in miRNA transfection experiments. The figure shows log₂ fold changes in mRNA levels (mRNA-seq), and ribosome-protected fragments (rpf) 12 and 32 h after hsa-miR-155-5p and hsa-miR-1 transfection. Changes in translation were estimated from the difference between changes in rpf and changes in mRNA levels. mRNAs with precisely one seed match to the transfected miRNA in the CDS and no seed match in the 3′ UTR were analyzed separately from mRNAs with precisely one seed match in the 3′ UTR and no seed match in the CDS. Fold changes were determined relative to the average fold change of mRNAs with no seed matches.