Jean Hausser; Afzal Pasha Syed; Biter Bilen; Mihaela Zavolan

Analysis of CDS-located miRNA target sites suggests that they can effectively inhibit translation

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 4.

mRNA destabilization occurs mainly through sites located in the 3′ UTR. Shown are log₂ fold changes in mRNA levels upon miRNA transfection in the experiments of (A) Linsley et al. (2007) and (B) Grimson et al. (2007). mRNAs with binding sites located in the CDS only, in the 3′ UTR only, and in both CDS and in 3′ UTR were analyzed separately. Fold changes were normalized to the average fold change of mRNAs that did not contain canonical binding sites to the transfected miRNA.