The A, B, and C cluster regions target distinct sets of transcriptional regulator encoding genes. (A) Regulatory network showing transcription factors targeted by CEBP-bound regions belonging to the A, B, or C clusters. The total number of connections (peaks associated with a gene) is indicated by color scale (white is one, and dark blue is 11, the maximum), and the relative, total expression (POL2 binding throughout the eight time points) is indicated by circle size. (B) CEBPB 3-h ChIP genomic coverage in the vicinity of three putative targets. Jun, Id2, and Egr1 loci display several CEBP peaks, i.e., “connections” in A. (C) Examples of putative feed-forward loops in the network. Arrows point from the transcription factor to the regulated gene. For clarity, the gene product level of CEBP targets has been left out. “A” and “C” are cis-regulatory elements belonging to the A or C cluster.
