The A, B, and C cluster regions target distinct sets of genes. (A) Heatmap of gene expression changes through liver regeneration. “Up” and “down” bins count genes associated with a CEBP cluster (A, B, or C), exceeding a 2.0-fold change threshold (POL2 binding level change relative to 0 h). Blue color intensity indicates number of genes going up; gray intensity, genes going down. (B) Representative examples of transcriptional activity (polymerase II [POL2] binding) of putative target genes. CEBPB binding illustrates gene association with either the C (Cps1) or A (Mdc1) cluster exclusively (upper left and right panel, respectively) or association with peaks belonging to both B and C clusters (Adh7 and Orm1; bottom panels). Gene loci are shown in black below; binding levels for CEBPB and POL2, in dark pink and red, respectively. Black numbers indicate POL2 gene body coverage for time point comparison. (Adh7) Alcohol dehydrogenase 7; for additional gene symbols, see Figure 1.
