A ChIP-seq time course of mouse liver regeneration. (A) Overview of the experimental time course. Eight time points covering the full week of the regenerative response; phases of the first cell cycle indicated. Previous studies show a prolonged decrease in the CEBPA to CEBPB ratio. (B) Experimental outline for ChIP experiments. Partial hepatectomy was carried out on five mice for each time point. The regenerating liver tissue was harvested after variable time intervals, fixed, and subjected to ChIP-seq. (C) Normalized coverage maps of four gene proximal regions with ChIP-seq peaks, marked by dashed vertical lines. Each row represents a time point for either CEBPA (top eight rows, dark purple) or CEBPB (bottom eight rows, dark pink). Gene loci are shown in black below. (D) CEBPA and CEBPB temporal binding profiles based on genomic coverage, generated from the peaks in the C. (Alb-enh) Albumin proximal region; (Mdc1) mediator of DNA damage checkpoint 1; (Orm1) orosomucoid 1; (Cps1) carbamoyl-phosphate synthetase 1.
