Highly active zinc-finger nucleases by extended modular assembly

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 5.
Figure 5.

Influence of “disruptive” interfinger linkers on extended-MA ZFN activity. ZFN activity was determined by the SSA assay for ZFNs with long linkers (TGSQKP) inserted between fingers 2 and 3 and fingers 4 and 5. (Std Link) All canonical linkers (TGEKP). The array configuration for the Std Link ZFN was GZF3-L3 + GZF1-R3, CS2-1 L5 + R3, CS5-1 L4 + R4, CS6-2 L5 + R4, CS7-3 L4 + R6, and T2-X6 L5 + R4.

This Article

  1. Published in Advance December 5, 2012, doi: 10.1101/gr.143693.112 Genome Res. 23: 530-538

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research