The ab initio B-score as a predictor of relative affinity and activity of MA ZFNs. (A) The B-scores are shown for each Barbas zinc-finger module (Bhakta and Segal 2010). B-scores were summed and compared to the measured affinities of (B) seven three-finger arrays (Sander et al. 2009); (C) 16 three-finger arrays (Segal et al. 1999); and (D) six six-finger arrays of mixed composition (MS Kim et al. 2011; Shimizu et al. 2011). (E) Receiver operating characteristic (ROC) analysis of four predictors of ZFN activity (≥8% SSA activity) was performed for the 92 array variants of the CS ZFN series using the Daim package of the R statistics program. (Comb.B) The combined B-score of the modules in the left and right arrays. (Comb.GNN) The combined number of GNN modules. (Comb.fns) The combined number of fingers. (Comb.ddG) The combined ΔΔG of the GNN modules (Sander et al. 2009).
