Highly active zinc-finger nucleases by extended modular assembly

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 3.
Figure 3.

Functional selection of ZFNs using the Surveyor assay in HEK293T cells. ZFN CS2-1-L5 + R3, as a positive control, and all 16 combinations of ZFN T2-X6 were assayed in duplicate. The number of fingers in the left and right arrays for each ZFN are indicated above each pair of lanes. Numbers below each lane indicate the percentage of modified alleles averaged over the duplicates. (Arrows) Appearance and position of the Surveyor cleavage band. (Black box) ZFN pair T2-X6 L5 + R4 that was used for Illumina sequencing analysis.

This Article

  1. Published in Advance December 5, 2012, doi: 10.1101/gr.143693.112 Genome Res. 23: 530-538

Preprint Server



Current Issue

  1. January 2026, 36 (1)
  1. Current Issue
  1. Alert me to new issues of Genome Research