Incorporation of RNA accessibility improves the identification of RBP targets. Shown are higher prediction accuracies for distinguishing true and false RBP targets than using motif count only evaluated on RIP-chip data sets of a number of RBPs with consensus motifs: (A) Msl5 with motif UACUAAC; (B) Puf3 with motif CNUGUAHAUA; (C) Puf3 with motif UGUAHAUA; (D) Puf4 with motif UGUAHMNUA; (E) Puf5 with motif WUUGUAWUWU; and (F) Yll032c with motif AAUACCY. The receiver operating characteristic (ROC) curves demonstrate the change of the true positive rate versus the false positive rate with varying cutoffs. The area under curve (AUC) of the ROC plot measures the discrimination accuracy. The higher the AUC, the better the discrimination accuracy is. The gray dashed line of the diagonal indicates no discrimination power (AUC=0.5).
