Constitutive nuclear lamina–genome interactions are highly conserved and associated with A/T-rich sequence

A/T content is a strong predictor of constitutive lamina association. (A) A/T content versus mean mouse lamin B1 association for a random sample of 10,000 defined cLAD, fLAD, and ciLAD regions (gray dots). (Black dots) The regions used for classification (1000 per group). (Dashed line) Genome-wide average (58%). (B) Tenfold cross-validation classification accuracies of classifiers using k-mers as predictive features, with k = 1 … 5. Bars show mean accuracies, with error bars indicating one standard deviation. (C) Log₂ ratios of occurrence counts in cLAD versus ciLAD regions for each dinucleotide. Bar shading indicates the A/T content of dinucleotides.