Irradiation-induced senescence does not result in SA-DNAm changes. (A) Representative growth curves of MSC after irradiation with different doses at passage 2 (0–25 Gy; black arrow). (B) Upon irradiation with 15 Gy, MSC acquire the typical senescent morphology with proliferation arrest and moderate SA-β-gal staining. Furthermore, adipogenic (green: BODIPY; blue: DAPI) and osteogenic differentiation potential (red: Alizarin Red; size bars 100 μm) is impaired after irradiation with 15 Gy. (C) Scatterplot analysis of DNAm of nonirradiated versus irradiated MSC. Long-term culture-associated hypermethylated (red) and hypomethylated CpG sites (green) are depicted. Irradiation did not result in any significant DNAm changes.
