Figure 3.
TERRA levels after knockdown of genes encoding candidate proteins. (A) Number of TERRA molecules in HeLa Kyoto transfected with the indicated esiRNA, quantified by qRT-PCR at 1q-2q-10q-13q (blue bars) and 15q (red bars), normalized to beta actin and compared to levels upon esiRNA transfection against RLuc. Error bars represent standard deviation (SD) between three independent experiments. (*) P < 0.05, (**) P < 0.005, (***) P < 0.0005. (B) Percentage of cells in G1 (blue), S (yellow), and G2–M (red) phase, assessed by flow cytometry after BrdU and propidium iodide labeling.
