Figure 1.
Schematic representation of the quantitative TERRA pull-down. (A) The nuclear extract of HeLa cells labeled by SILAC was incubated with immobilized UUAGGG-repeat-containing RNA or a shuffled control fragment. Both fractions were combined prior to quantitative mass spectrometry. (B,C) Two-dimensional interaction plot for the TERRA pull-down under relatively mild (250 mM sodium chloride) washing conditions (B) and under more stringent (1 M sodium chloride) washing conditions (C). The 15 candidates (red-filled circles) selected for the secondary RNAi screen are annotated.
