Directed shotgun proteomics guided by saturated RNA-seq identifies a complete expressed prokaryotic proteome

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Figure 2.
Figure 2.

Transcriptome coverage by RNA-seq. (A) Saturated coverage of protein-coding genes. An estimate of the number of actively expressed protein-coding genes based on the number of uniquely mapped RNA-seq reads is shown for both conditions and biological replicates. (B) Density distribution of RPKM values. In addition, boxplots representing the expression level of the 11 members of the virB/D4 operon are shown for the uninduced (black), and induced (red) condition. For clarity, we only show data for the sample pair uninduced2/induced2.

This Article

  1. Genome Res. 23: 1916-1927

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