Figure 1.
Construction and analysis of a miRNA precursor intermediates library (SPARE). (A) Scheme illustrating the SPARE library construction used to map processing intermediates and the expected results depending on the processing direction of the miRNA precursors; (1) indicates the first cleavage reaction and (2) the second one. (B) Modified 5′ RACE PCR results from wild-type and fiery1 mutants on selected miRNA precursors. (C) Scheme of the procedure to analyze the SPARE data. (D) Frequency of detected cuts flanking the miRNA/miRNA* region (green) and other positions along the precursor (purple).
