Lamin A/C-promoter interactions specify chromatin state–dependent transcription outcomes

LMNA interacts with promoters in adipose stem cells. (A) Immunofluorescence detection of lamin A/C in ASCs. (Inset) Phase contrast. Bar, 10 μm. (B) Western blot analysis of lamin A/C in ASCs, ASC chromatin (input), and antibody-unbound and -bound fractions after LMNA ChIP. (C) Browser view of LMNA occupancy on two regions of chromosome 1; (left) nucleotide 1,230,000–1,260,000 (TNFRSF18, TNFRSF4, SDF4, B3GALT6); (right) nucleotide 12,810,000–12,888,000 (PRAMEF11, HNRNPCL1, PRAMEF2, PRAMEF4, PRAMEF12) in ASCs before and after LMNA KD. (D) Number of genes with a LMNA-associated promoter in ASCs and in LMNA-KD ASCs. (E) GAGA and A/T-rich motifs enriched in promoter regions underlying LMNA peaks.