Subcellular localization of N- and C-terminal FLAG-tagged LRRC37A. (A) HeLa cells transiently transfected with N- and C-terminal FLAG-tagged LRRC37A constructs and probed with anti-FLAG antibody. LRRC37A tagged at the N-terminus does not show strong localization, whereas LRRC37A tagged at the C-terminus shows a plasma membrane localization (magnification, 63X). (B) Western blot of lysates and conditioned media of HeLa cells transfected with FLAG-tagged LRRC37A. Lysates and conditioned media of HeLa cells transiently transfected with N-terminal (N) or C-terminal (C) FLAG-tagged LRRC37A, probed with anti-FLAG (lanes 1–3) and anti-LRRC37 (lanes 4–6) antibodies. Nontransfected HeLa cells represent the control. (C) Conditioned media were concentrated, and a comparable amount of lysates and media was loaded. Samples were probed with anti-LRRC37 antibody.
