CRY2 deficiency disrupts circadian behavior. (A) The act of monarch eclosion from chrysalis to butterfly. (B) Profiles of adult eclosion in constant darkness (DD) of wild-type (+/+, black), heterozygous (+/−, gray), and homozygous mutant (−/−, red) siblings of the 4-bp deletion (left) and the 2-bp insertion (right) cry2 mutant line, entrained to LD throughout their larval and pupal stages. Eclosion occurred on the first day and second day of DD. Data from both days are pooled and binned in 1-h intervals (for detailed data throughout the 2 d in DD) (see Supplemental Fig. S3). Effect of genotype on eclosion time, one-way ANOVA: P < 0.0001 for both lines; Tukey post-hoc test: +/+ versus +/−, P > 0.05; +/+ versus −/−, P < 0.01; +/− versus −/−, P < 0.01 for both lines. (Black horizontal bars) Night/subjective night; (gray horizontal bars) subjective day.
