ChIP-seq guidelines and practices of the ENCODE and modENCODE consortia

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Figure 1.
Figure 1.

Overview of ChIP-seq workflow and antibody characterization procedures. (A) Steps for which specific ENCODE guidelines are presented in this document are indicated in red. For other steps, standard ENCODE protocols exist that should be validated and optimized for each new cell line/tissue type or sonicator. (*) A commonly used but optional step. (B) Flowchart for characterization of new antibodies or antibody lots. (C) Flowchart for use of antibody characterization assays.

This Article

  1. Genome Res. 22: 1813-1831

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