Figure 8.
Sensitivity of the RT-PCR-seq method. The RT-PCR-seq validation rates of exon–exon junctions are shown as a function of the abundance of the targeted transcript isoforms. The latter was ascertained by counting the number of validating reads detected in HBM RNA-seq experiments. The results obtained for the seven tissues used in both HBM RNA-seq and GENCODE RT-PCR-seq were considered (brain, heart, lung, testis, liver, kidney, and skeletal muscle) either separately (A) or merged together (B).
