Examples of genBlastG-revised C. briggsae gene models. (A) Revision: The WormBase C. briggsae gene model CBG09754 has three exons and two introns. The genBlastG-revised gene model, JNC_CBG06430, has two new exons incorporated. Protein sequence encoded by the revised C. briggsae gene model displays very high PID (99.1%) with the protein sequence encoded by its C. elegans ortholog ben-1/tbb-5 (C54C6.2). ben-1 encodes a redundant beta-tubulin in C. elegans and it is the only benzimidazole-sensitive beta-tubulin in C. elegans (Driscoll et al. 1989). (B) Merge: Based on homology, three WormBase C. briggsae gene models (CBG08435, CBG08437, and CBG08438) were merged to form a single-gene model JNC_CBG02055, which is orthologous with C. elegans gene lrk-1 (T27C10.6) with high identity (92.63%). LRK-1 is the C. elegans ortholog of the familial Parkinsonism gene LRRK2 (previously known as PARK8) that is required for polarized localization of synaptic vesicle (SV) proteins (Sakaguchi-Nakashima et al. 2007). (C) Split: Based on homology, a single WormBase C. briggsae gene model CBG09729 is split into three separate gene models JNC_CBG16717, JNC_CBG16718, and JNC_CBG16719, which are homologous with three fully confirmed C. elegans gene models F32D8.14 (PID = 91.63%), F32D8.4 (PID = 70.55%), and F32D8.12a (PID = 90.11%), respectively. (D) Novel C. briggsae gene model: Based on homology, genBlastG builds a novel C. briggsae gene model JNC_CBG14647, which is orthologous with the fully confirmed C. elegans gene model F54F4.16 (PID = 90.62%).
