Figure 4.
Mapping of malbec. (A) Homozygosity scoring maps the mutation to chromosome 7. (B) Log likelihood calculation reveals a ∼23-Mb high-scoring interval. (C) Meiotic map of the mlb locus on chromosome 7. The tightly linked genetic marker snp1 was identified by a traditional chromosomal walk. (D) NGS sequence analysis of mlb and control embryos identified a nonsense mutation in a candidate gene disrupted in mlb. This nonsense mutation is nonrecombinant with the mutant phenotype and resolved all remaining 12 genetic recombinants to a resolution <1/5440 meioses.
