Figure 6.
Consequence of editing at the +4 site in miR-381 throughout development. (A) Editing of pri-miR-381 at five different developmental stages. RNA from whole mouse brain was amplified by RT-PCR and sequenced. The different developmental stages used for sequencing are indicated in the figure. (Arrow) The edited position. Editing of miR-381 in RNA extracted from an ADARB1-null mouse (A2−/−) indicates that ADAR is the major enzyme to edit this site. (B) Detection of Pum2 mRNA levels using semiquantitative RT-PCR. RNA from whole mouse brain at five different developmental stages (E15, E19, P0, P7, and adult) was amplified by RT-PCR with primers specific for Pum2.
