Editing of miR-376a redirects targeting during development. (A) Editing of pri-miR-376a at six different developmental stages. RNA from whole mouse brain was amplified by RT-PCR and sequenced. The different developmental stages used for sequencing are as indicated. (Arrows) Edited positions. (B) RNA from whole mouse brain at four different developmental stages (E15, P2, P7, and adult) was amplified by RT-PCR, and mRNA levels of TTK and PRPS1 were analyzed by qPCR. All error bars represent the SEM. (C) Protein levels of PRPS1 during development of the mouse brain. Immunoblot analysis showing PRPS1 protein expression during developmental day E19, P2, P7, P14, and adult. An anti-actin antibody was used as loading control.
