mRNAs containing C-rich 3′ UTR elements are destabilized in iPS cells and poly(C)-binding proteins are differentially expressed. (A) Table of hexamers over-represented in the 3′ UTR of transcripts that were significantly less stable in iPS cells than in HFFs. (B) Transcripts that have C-rich elements in their 3′ UTRs are less stable in iPS cells. Half-lives of DGCR8, DUSP7, and TOB2 mRNAs were determined by qRT-PCR following inhibition of transcription with actinomycin D. The error bars represent the standard deviation based on three experiments. (C) Western blots showing abundance of poly(C)-binding proteins PCBP1, PCBP2, PCBP3, and PCBP4 in HFF and iPS cells. Abundance was normalized to GAPDH or alpha-tubulin. The numbers beneath each blot represent the relative amount of each protein in iPS cells with standard deviation derived from three independent experiments.
