mRNAs containing U-rich 3′ UTR elements are destabilized in iPS cells and RBPs that recognize these types of sequence are differentially expressed. (A) mRNA decay was assessed by qRT-PCR following inhibition of transcription with actinomycin D. Each graph represents the average of three experiments. The error bars denote the standard deviation. (B) Western blots showing relative abundance in HFF and iPS cells of various RNA-binding proteins shown to interact with U-rich sequences. The numbers below each blot represent the relative amount of the protein after normalization to alpha-tubulin. Relative abundance of PTBP1 cannot be assessed, as it was undetectable in the HFF cells.
