KRAB C2H2-type zinc finger protein mRNAs are stabilized in iPS cells. (A) Box-and-whisker plot showing fold change in half-life between HFF and iPS cells for different subsets of transcripts. (All genes) The entire data set of 5481 mRNAs; (C2H2 ZNF) 532 transcripts linked with the SMART term C2H2 ZNF (SM00355); (KRAB) 263 transcripts associated with the SMART term KRAB (SM00349). P-values were derived by the Kolmogorov-Smirnov Test. (B) Graphs showing decay of ZNF43, ZNF134, and ZNF627 mRNAs in HFF and iPS cells following inhibition of transcription with actinomycin D. mRNA abundance at each time point was determined by qRT-PCR. The error bars denote standard deviation based on three replicates. (C) Graph showing abundance of ZNF-targeting miRNAs in HFF and iPS cells as measured by qRT-PCR. Error bars represent the standard deviation derived from three independent experiments.
