Figure 1.
Design of a cDTA experiment. The Sc cells are labeled by adding 4tU into the media, whereas Sp cells are labeled by adding 4sU. The cells are then counted. Sc cells from different experiments are always mixed with the same amount of labeled Sp cells from a single batch. Cells are then lysed, RNA is extracted, biotinylated, and labeled RNA separated. Microarrays containing probes against both Sc and Sp transcripts are then used to quantify both total and labeled RNA.
