Nickase-mediated genomic deletions in human cells. (A) Schematic representation of ZFN-mediated genomic deletions. (B) PCR products corresponding to the 15-kb genomic deletions in cells transfected with plasmids (4 μg/monomer) encoding nucleases or nickases. (The black triangle above the gel picture) The increase of the transfected plasmid (4, 8, and 10 μg/each monomer). (C) DNA sequences of deletion PCR products that were amplified from genomic DNA isolated from cells transfected with a single nickase pair. Nuclease target sites are shown in boldface letters. Mismatched bases between the CCR2 and CCR5 loci are indicated as lowercase letters. Mutated bases are shown in italics. The regions in which recombination between the CCR2 and CCR5 loci occurred are underlined.
