Precision genome engineering with programmable DNA-nicking enzymes

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Figure 5.
Figure 5.

Mutagenic NHEJ frequencies at on-target and off-target sites. K562 cells were transfected with plasmids encoding ZFN-224, the ZF nickase (L_KK/R_el), or an empty vector used as a negative control. PCR amplicons corresponding to the CCR5 on-target site and 13 off-target sites were subjected to high-throughput sequencing. Sequences that contained indels within the spacer region were considered to be NHEJ-mediated modifications.

This Article

  1. Published in Advance April 20, 2012, doi: 10.1101/gr.138792.112 Genome Res. 22: 1327-1333

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