Figure 2.
Comparison of activities of ZFNs and ZF nickases using a cell-based reporter system. (A) Schematic overview of a single-strand annealing (SSA) system. (B) Measurement of nuclease and nickase activities using the cell-based SSA system. Means and standard deviations (error bars) from at least three independent experiments are shown. P-values were calculated with the Student's t-test; (*) P < 0.05 (empty vector vs. nickase).
