Transcripts differentially regulated by period. Two-way ANOVA was used to identify transcripts differentially regulated by per0 in both non-poly(A)- (A) and poly(A)-amplified (B) data sets. Median-normalized expression levels have been sorted by fold change (FC) (average wild-type expression in RPKM divided by average per0 expression in RPKM) and are represented as a heatmap for each transcript (vertical axis) at each time point (horizontal axis) surveyed, with yellow indicating high levels of expression, and blue, low levels. White and black bars represent light and dark, respectively, in the LD environmental regimen under which these samples were collected. The order of transcripts along the vertical axis is identical in A and B. Tequila-RB (C) and Cyp4p1-RA (D) are examples of transcripts whose expression is significantly altered between wild-type (blue) and Period loss-of-function (red). (Error bars) Mean ± standard error of the mean.
