X-linkage, expression breadth, and sex-biased expression of D. melanogaster genes. (Barplots) Percent of D. melanogaster genes in various expression classes that are X-linked. (Dashed lines) Percent of all genes within that panel that are X-linked. (*) Observed values that significantly differ from the expectation based on the size of the X chromosome as determined by a permutation test; (*) P < 0.05, (***) P < 0.001. (A) Percent of broadly or narrowly expressed genes with either female-biased (F), male-biased (M), or unbiased (U) expression is plotted. (B) Percent of genes with unbiased expression that are either broadly (τ ≤ 0.4) or narrowly (τ > 0.7) expressed is plotted. Genes were assigned to sex-biased expression classes using RNA-seq data (BWA alignments and edgeR to call differential expression).
