A chimera with confirmed RNA and protein expression. We detected two overlapping unique peptides that matched the junction site in 18 mass spectrometry experiments and by the targeted mass spectrometry (SRM) analysis, confirming that this transcript (ESTid = “BM838228.1”) from ChimerDB (Kim et al. 2010) is expressed at the protein level. (A) The 3D structure of the chimeric protein is modeled by Phyre2 (Kelley and Sternberg 2009). (Green) The chimeric protein part derived from actin, ACTG1, predicted using homology modeling; (red) the part of the ribosomal protein, RPL13A, predicted using ab initio methods. The structure is modeled using the Ribonuclease H-like motif fold (actin-like ATPase domain) with 100% confidence and 85% identity. (B) The secondary structure modeling by Phyre2 (Kelley and Sternberg 2009) predicts that a highly preserved beta strand appearing in the wild-type actin protein should also feature in the chimera (blue rectangle). The motif “GDGV” (red rectangle) is the ATP-binding site, which is missing in the chimera sequence.
