Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 1.
Figure 1.

BisChIP-seq DNA methylation profiles of H3K27me3-enriched DNA from normal PrEC and cancer LNCaP cells. (A) Flowchart of BisChIP-seq protocol to perform bisulfite treatment and library preparation on H3K27me3-ChIP DNA. (B) Distribution of H3K27me3-enrichment genome-wide relative to observed over expected and pie charts showing relative distributions across the genome. (C) Affymetrix Gene 1.0 ST expression values for H3K27me3-marked and -unmarked genes in PrEC and LNCaP cells. (D) Distribution frequency of CpG methylation levels at H3K27me3-marked regions that fall into each regional annotation category from low (0%) to high (100%) methylation (0.0–1.0).

This Article

  1. Published in Advance March 30, 2012, doi: 10.1101/gr.132076.111 Genome Res. 22: 1120-1127

Preprint Server



Current Issue

  1. March 2026, 36 (3)
  1. Current Issue
  1. Alert me to new issues of Genome Research