Nucleosomal DNA properties of Dictyostelium nucleosomes. (A) 73,396 nucleosome dyad locations were grouped as −1 (N = 3230), +1 (N = 7285), and all other genic nucleosomes (N = 63,643) (see diagram). For the three assigned nucleosome groups, the W (= A/T in black) and S (= G/C in red) nucleotide percentage at each position was calculated on the sense strand (5′ → 3′ from left to right), so as to maintain directionality of the frequency patterns with respect to the TSS. Plots were smoothed via a 3-bp moving average. (Gray and light red) Randomized distributions. The schematic bar in the upper part represents the rotational orientation of the major groove against the histone octamer surface. (B) Frequency distribution of the WW, SS, and SW+WS dinucleotides relative to nucleosome dyads, as described in Albert et al. (2007) and with the same randomization.
