The construction of a PAC library from the genomic DNA of the wild-type parasite. (A) A schematic drawing of PACv1. (B) The PACv1 library was made from the genomic DNA of wild-type parasites and directly introduced into these parasites by electroporation. The parasitemia was monitored every 24 h after transfection. The arrow indicates the initiation of the drug treatment of the parasites. (C) Southern blot analysis of PACv1 (including DNA fragments) was performed using the hdhfr gene as the probe. (Lanes C and L) Linearized PACv1 without an inserted DNA fragment and the PAC library of the total population of transfected parasites, respectively. (Lane 1) Parasites with self-ligated PACv1. The remaining 13 lanes show parasites with PACv1 (including DNA fragments).
