
Ethanol tolerance of the Brazilian bioethanol production strain VR1 and its segregant VR1-5B. The ethanol tolerance of VR1 (diploid) and VR1-5B (haploid) was determined by scoring growth of tenfold dilutions on YP plates with different concentrations of ethanol. Both strains, as well as the heterozygous VR1-5B/BY4741 strain (diploid) showed a clearly higher ethanol tolerance than the control laboratory strains BY4741 (haploid) and BY (diploid), the latter of which was obtained by crossing BY4741 with BY4742.











