Identification of novel causative genes determining the complex trait of high ethanol tolerance in yeast using pooled-segregant whole-genome sequence analysis

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Figure 1.
Figure 1.

Ethanol tolerance of the Brazilian bioethanol production strain VR1 and its segregant VR1-5B. The ethanol tolerance of VR1 (diploid) and VR1-5B (haploid) was determined by scoring growth of tenfold dilutions on YP plates with different concentrations of ethanol. Both strains, as well as the heterozygous VR1-5B/BY4741 strain (diploid) showed a clearly higher ethanol tolerance than the control laboratory strains BY4741 (haploid) and BY (diploid), the latter of which was obtained by crossing BY4741 with BY4742.

This Article

  1. Genome Res. 22: 975-984

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