Typical BRIC-seq data. (A) Typical mapping data of RNA-seq tags obtained from BRIC-seq using a Genome Analyzer (Illumina). The chromosomal locus of AK091718 is shown. (B) The relative level of AK091718 RNA remaining was determined by BRIC through deep sequencing. The numbers of sequencing tags at the various time points are shown in A. The relative quantitative values at time 0 h were arbitrarily adjusted to 100%. (C) The expression of AK091718 in HeLa TO cells assessed by Northern blot hybridization. (D) Distribution of RNA half-lives determined by BRIC-seq. BRIC-seq determined the half-lives of 11,052 mRNAs and 1418 ncRNAs expressed in HeLa TO cells. (Black circles) The fraction of mRNAs; (white circles) the fraction of ncRNAs. Transcripts with a half-life >24 h are included in the category “>24.”
