Nuclei purification enriches for muscle genes down-regulated in an unc-120 mutant. Nuclei were isolated from unc-120(st364) and wild-type background, and RNA from total nuclei and affinity-purified muscle nuclei was analyzed using high-density tiling arrays. Averages of four biological replicates of wild type and two biological replicates of unc-120 were used for the analysis. All 20,301 genes are ranked from muscle-enriched to muscle-depleted according to their difference in expression between total and muscle nuclei. T-values reflecting the differences between wild-type and unc-120 background are plotted on the y-axis. The top panel compares total nuclei and the bottom panel compares muscle nuclei. Genes with significantly reduced expression in unc-120 background are shown in blue (q < 0.01). The red line marks the cutoff for genes significantly enriched in muscle nuclei (q < 0.01).
