Sowah intronic CNRs. Two of the Sowah2 pre-WGD CNRs that function as tissue-specific enhancers in reporter assays (represented by red ovals and rectangles in Fig. 6). (Left) CNR 54390 (Sowah2 intron 7); (right) CNR 3240 (Sowah2 intron 8). (A) Sequence alignment of the 54390 and 3240 Sowah-CNRs in different Irx complexes of several species. Shadowed nucleotides correspond to >60% sequence conservation. (B,C) GFP expression driven by the elements 54390 and 3240. Paralogous sequences of the element 54390 in complexes IrxA and IrxB drove similar expression patterns in Xenopus embryos (C) and zebrafish (B) transgenic lines. In the case of the CNR 3240, only that present in the IrxA complex was found to be positive in transgenesis studies. (e) Eye; (m) midbrain; (h) hindbrain; (s) spinal cord.
