DLG4 and SYP expression during PFC development. (A,C) Western blots conducted, respectively, using SYP- and DLG4-specific antibodies in PFC samples of individuals of different age in the three species. The age of each individual is shown above the lanes (m, months; d, days; y, years). DLG4 and SYP bands were observed at the predicted molecular weight of 95 kDa and 38 kDa. An equal amount of protein was loaded to each lane. ACTB (also known as beta actin) and TUBB3 (also known as Tubulin beta-III) were used as loading controls (bands at the predicted weight of 42 kDa and 55 kDa). (B,D) Protein expression level of SYP and DLG4 in PFC development measured as the integrated optical density (IOD) from the Western blots bands (gray bars) and measured using published mass-spectrometry–based protein time-series (red lines) (Fu et al. 2011), as well as mRNA expression levels measured using microarrays in this study (blue line). The y-axes show the expression values across samples. The axis' colors correspond to the measurements: gray, Western blot; red, mass spectrometry; and blue, microarrays.
