EBF, TBP, and MEF2A proteins are expressed in the MOE and physically associate with the promoters of Olfr110, Olfr279, Olfr683, Olfr794, or Olfr1106 in vivo. (A) Immunohistochemistry of MOE from mice at postnatal day 21, with anti-EBF, anti-TBP, and anti-MEF2A antibodies (green). Nuclei were visualized with DAPI (blue). Scale bars, 30 μm (top row), 60 μm (center and bottom row). (B) ChIP assays were performed on MOE from mice at 22–30 d postnatally. Chromatin was immunoprecipitated with anti-EBF, anti-TBP, and anti-MEF2A antibodies. Normal rabbit IgGs were used as negative control. Enrichment of promoter sequences in the immunoprecipitates was determined by qPCR, using specific primers. The relative abundance of target DNA was expressed as percentage of total input chromatin. Data were obtained from two independent experiments. Statistical analysis: p < 0.05 in all cases.
