Replication studies of the KIF5B-RET fusion gene in an additional five triple-negative lung adenocarcinomas. (A) cDNA PCR targeting KIF5B-RET fusion transcripts and gel electrophoresis in the liver metastatic lung cancer of AK55 and five additional triple-negative lung adenocarcinomas. cDNA from AK55 and LC_S2 shows clear evidence of the fusion transcript. Because the fusion transcript in AK55 contains one more exon of KIF5B (exon 16) compared with that in LC_S2 (exon 15), the size of the PCR product in AK55 is longer than that in LC_S2. (B) cDNA PCR targeting KIF5B-RET fusion transcripts and gel electrophoresis in 15 double-negative lung adenocarcinomas. LC_S6 shows clear evidence of the fusion transcript. The fusion transcript in LC_S6 contains seven more exons of KIF5B (exons 17–23) compared with that in AK55. (C) Comparison of schematic KIF5B-RET fusion transcripts between AK55, LC_S2, and LC_S6. Each rectangle indicates an exon of KIF5B (blue) and RET (red) gene.
