
Detection of amplification at two CNV intervals in tumor DNA. Real-time qPCR was used to analyze locus-specific assays that map within the CNVs at 4q13.2 and 16p12.3 using unamplified template DNA. Each amplicon was measured relative to the mean of four reference loci, by relative quantitation. Unamplified tumor DNA from 37 primary breast cancers (from an independent series) was compared with 56 normal leukocyte DNA samples. Amplification (RQ > 2.5) was detected in tumor DNA only.











