Endo-siRNAs mapping antisense to lincRNAs. (A) Abundance of endo-siRNAs mapping antisense to 73 lincRNAs with mean RPKM ≥ 1. The key indicates the log-scaled RPKM values (endo-siRNA reads per kilobase per million genomic mapping reads). The lincRNAs were sorted by the mean RPKM values (averaging RPKMs calculated from all 35 RNA-seq samples). The data used to make this heat map are presented in Supplemental Table S6. (B) Improved annotations of loci corresponding to the top 30 22G-RNA clusters from the adult stage. (Left panel) Fractions of 22G-RNAs mapping to the antisense strand (red), sense strand (green), and intergenic or intronic regions (gray) of protein-coding genes annotated in ce6. (Right panel) Fractions of 22G-RNAs mapping to the indicated transcripts of the de novo gene annotation, highlighting those mapping antisense to new transcripts (orange). Clusters mapping antisense to either lincRNAs or newly annotated transcripts that satisfied only two of the three lincRNA filtering criteria are indicated (blue and gray asterisks, respectively) as are those mapping antisense to pseudogenes (T09F5.12, Y39E4B.14, and C47G2.6). (C) Improved annotations of loci corresponding to the top 30 26G-RNA clusters from the embryo stage; otherwise, as in B.
