Inhibition of DNA methylation results in restoration of gene expression for IDH1R132H/WT-repressed loci. (A) Candidate gene expression reactivation in 5-aza-2′-deoxycytidine-treated IDH1-mutant cell lines. Parental or IDH1R132H/WT knock-in (KI-1, KI-2) cells were treated with 5 μM of 5-aza-2′-deoxycytidine (DAC) or control (PBS) for 48 h. Following treatment, relative mRNA levels of UBB, RBP1, and SERPINB5 were measured by Q-PCR. (Bar graph) Expression fold-change relative to untreated parental cell line. (Error bars) Standard deviation of three independent experiments. (B) Bisulfite sequencing analysis of candidate genes in A for untreated and DAC-treated cell lines. Each row represents a sequenced allele and each dot represents a CpG. (Black dots) Methylated CpGs; (white dots) unmethylated CpGs.
