A heterozygous IDH1^R132H/WT mutation induces genome-wide alterations in DNA methylation

Global and gene-specific histone lysine methylation coincides with IDH1^R132H/WT-induced DNA methylation. (A) Western blots for total H3K4me3, H3K9me3, H3K27me3, and H4K20me3 histone modifications for parental (HCT116) and IDH1^R132H/WT knock-in (KI-1, KI-2) cell lines. Also shown are total H3 and H4 controls. (B) ChIP was performed using antibodies against H3K9me3, H3K27me3, pan-H3, or IgG and immunoprecipitated DNA quantified by Q-PCR using primers specific to the promoter regions of the indicated genes. (Bar graphs) The mean enrichment of the specific histone mark relative to that of total histone H3 for the same genomic region. (Error bars) Standard deviation of two independent experiments except for PDLIM2.