BEAF-32-associated close head-to-head genes are not coexpressed. (A) Distribution of the correlation of expression for the two genes in close head-to-head gene pairs (distance <500 bp). (Red arrow) Secondary peak for enrichment of genes that are not coregulated. (B) Percentage of gene pairs associated or not associated with BEAF-32 binding sites present between coexpressed and non-coexpressed genes in close head-to-head gene pairs. (C) Examples of BEAF-32 location in coexpressed and non-coexpressed gene pairs. The blocks indicate genes with FlyBase IDs. Blocks on top of the track are transcribed from the plus strand, and blocks at the bottom of the track are transcribed from the minus strand. The tracks under the gene tracks show the location of BEAF-32 signal with raw reads from ChIP-seq. The symbol “co-ex” represents the level of coexpression between the two genes. Detailed information about the expression of these genes is presented in Supplemental Table S2.
