Figure 6.
Insulator proteins, including MOD(MDG4), are necessary for the maintenance of H3K27me3 levels at several loci. (A–C) ChIP-seq profiles for insulator proteins and H3K27me3 levels before and after dCTCF knockdown at the even skipped, eyes absent, and Ods-site homeobox gene loci, respectively. (D–F) ChIP–PCR levels determined by qRT–PCR against H3K27me3 ChIP before and after knockdown of several insulator proteins. Control sample is represented as SEM from three independent biological replicates.
